The popular whole LC time is 6 h (the good thing about a longer LC time is smaller). For mouse tissues, starting substance consisting of a hundred μg of protein before enrichment was acceptable for only one LC-MS/MS run. We also analyzed the reproducibility concerning a number of runs. https://paxtonltyvu.pages10.com/the-single-best-strategy-to-use-for-how-long-to-deplete-glycogen-67122292